
Thermo Fisher Scientific Human FGF-17 Recombinant Protein
인간 FGF-17 재조합 단백질로, 세포 성장 및 발달 연구용으로 사용됩니다. E. coli 발현 시스템에서 생산되며 ≥95% 순도와 낮은 엔도톡신 수준을 보유합니다. 생물학적 활성은 Balb/c 3T3 세포 증식으로 검증되었습니다. 장기 보관 시 -20°C 이하에서 안정적입니다.
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Thermo Fisher Scientific Human FGF-17 Recombinant Protein
Applications and Tested Dilution
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human FGF-17 recombinant protein contains 195 amino acids |
| Molecular Weight | 22.7 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥95% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 10 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 10 mM Tris, pH 7.4, with 30 mM NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Reconstitution:
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute in 5 mM Tris, pH 8.0, to a concentration of 0.1–1.0 mg/mL. Do not vortex. Store the solution at 2–8°C for up to 1 week. For extended storage, dilute in a buffer containing 0.1% BSA and store aliquots at -20°C to -80°C.
Storage:
Lyophilized protein is stable at room temperature for 1 month but should be stored at -20°C for long-term stability. Working aliquots with carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
The FGF-17 protein belongs to the fibroblast growth factor (FGF) family, known for mitogenic and cell survival activities. FGF family members are involved in embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF-17 is highly expressed in the cerebellum and cortex. In mice, the homolog is found in midline structures of the forebrain, midbrain-hindbrain junction, developing skeleton, and arteries, indicating roles in CNS, bone, and vascular development.
This gene was previously referred to as FGF-13, but its amino acid sequence and chromosomal localization are identical to FGF-17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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