
Thermo Fisher Scientific Human FGF-9 Recombinant Protein
Human FGF-9 재조합 단백질로, 세포 성장 및 신경계 발달 연구에 적합합니다. E. coli 발현 시스템에서 생산되며 ≥95% 순도와 낮은 엔도톡신 함량을 보장합니다. Balb/c 3T3 세포 증식 활성 검증 완료. 다양한 생리학적 및 발달 연구용.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human FGF-9 recombinant protein contains 206 amino acids |
| Molecular Weight | 23.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥95% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 10 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Reconstitution:
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 0.1–1 mg/mL. Do not vortex. Store the solution at 2–8°C for up to 1 week. For extended storage, dilute further in buffer containing a carrier protein (e.g., 0.1% BSA) and store aliquots at -20°C to -80°C.Storage:
The lyophilized protein is stable at room temperature for 1 month but should be kept at -20°C for long-term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in various biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion.
FGF-9 was isolated as a secreted factor that stimulates growth of cultured glial cells. In the nervous system, it is mainly produced by neurons and may play a role in glial cell development. Expression of the mouse homolog depends on Sonic hedgehog (Shh) signaling; mice lacking the homolog exhibit male-to-female sex reversal, indicating involvement in testicular embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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