Thermo Fisher Scientific Human FGF-10 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human FGF-10 recombinant protein contains 170 amino acids

Molecular weight

19.3 kDa

Class

Recombinant

Type

Protein

Purity

≥95% by SDS-PAGE and HPLC

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 = 0.5 - 15 ng/mL; determined by the dose-dependent proliferation of 4MBr-5 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

5mM sodium phosphate with 80mM NaCl

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 5 mM sodium phosphate, pH 7.4, to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1-1.0% BSA

Storage: The lyophilized protein is stable at room temperature for up to 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

Target Information

FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This protein is also implicated to be a primary factor in the process of wound healing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.