Thermo Fisher Scientific BrdU Monoclonal Antibody (MoBU-1)
BrdU 및 BrU에 특이적으로 반응하는 단클론 항체로, EdU와 교차 반응하지 않음. IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능. 세포 증식 연구 및 DNA 복제 탐지에 적합. 4°C 보관, Protein A 정제.
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Applications 및 Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | 1 publication |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | 1 publication |
| Flow Cytometry (Flow) | 1–2 µg/mL | 3 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MoBU-1 |
| Immunogen | BrdU conjugated to hemocyanine |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1070849 |
Product Specific Information
This antibody reacts specifically with 5-bromouridine (BrU) and does not cross-react with EdU.
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside and an analogue of thymidine. BrdU is widely used to detect proliferating cells in living tissues. It incorporates into newly synthesized DNA of replicating cells during the S phase, substituting for thymidine during DNA replication. Antibodies specific for BrdU can then detect incorporated BrdU, indicating actively replicating cells. Detection requires DNA denaturation, typically by acid or heat treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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