Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (TU-16)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL, 60 min

-

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Many, Mouse, Pig, Rat

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TU-16

Immunogen

Porcine brain Microtubule protein MTP-1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Tubulin,uniProtId:Q2XVP4-1,ncbiNodeId:9821,antigenRange:1-451,antigenLength:451,antigenImageFileName:MA1-19209_alpha_Tubulin_Q2XVP4-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19209_alpha_Tubulin_Q2XVP4-1_House_mouse_PDP.jpeg,sortOrder:10},{targetFamily:alpha Tubulin,uniProtId:P02550-1,ncbiNodeId:9821,antigenRange:1-451,antigenLength:451,antigenImageFileName:MA1-19209_alpha_Tubulin_P02550-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19209_alpha_Tubulin_P02550-1_House_mouse_PDP.jpeg,sortOrder:9}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

TBS, pH 8

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_1070256

Product Specific Information

This antibody reacts with alpha-tubulin of all tested species, under denaturing and non-denaturing conditions.

Immunoprecipitation: Reducing conditions.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.