Thermo Fisher Scientific CD80 Monoclonal Antibody (MEM-233)
CD80 (B7-1) 단백질의 세포외 도메인을 인식하는 마우스 단클론 항체로, 인간 시료에 반응합니다. Flow cytometry, ICC/IF, IP 등 다양한 응용에 사용 가능하며, Protein A로 정제된 액상 형태입니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1–4 µg/mL | View 12 publications |
| Immunoprecipitation (IP) | Assay-dependent | View publications |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-233 |
| Immunogen | Extracellular domain of human CD80 fused to human IgG1 (Fc) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1073446 |
Available Conjugated Formats
Product Specific Information
This antibody reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of the immunoglobulin supergene family. CD80 is expressed on professional antigen-presenting cells such as dendritic cells, macrophages, or activated B lymphocytes.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of the T cell costimulatory molecule CD28 and the inhibitory receptor CTLA-4 (CD152). Both are expressed on antigen-presenting cells and are essential for T cell activation.
CD80 is rapidly induced on the surface of in vitro activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes.
The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes via CD28 and CTLA4 binding.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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