Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), NovaFluor Blue 760, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 0.2 µg/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV Series, Brilliant Violet Series, eFluor Series, FITC, Functional Grade, NovaFluor Blue Series, NovaFluor Red Series, NovaFluor UV/Violet/Yellow Series, PE, PE-Cyanine Series, PE-eFluor 610, PerCP-eFluor 710, Super Bright Series.
Custom conjugation available upon request.

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells, playing a key role in T cell development and activation. The GK1.5 binding is blocked by RM4-5.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; use ≤0.2 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment with NovaFluor dyes.
• This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which must be used for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).
• NovaFluor conjugates use Phiton™ technology, providing engineered fluorescent signatures with minimal spillover and spread.

Excitation: 492 nm
Emission: 766 nm
Laser: 488 nm (Blue)

Target Information

CD4 is a co-receptor for MHC class II molecules, primarily expressed on T helper cells, and also found on monocytes, macrophages, and dendritic cells. It plays a central role in T-cell activation via association with the T-cell receptor complex and Lck kinase. CD4 expression is observed in thymus, lymph nodes, tonsils, spleen, and various non-lymphoid tissues. It may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been reported (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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