Thermo Fisher Scientific beta Actin Polyclonal Antibody
Rabbit polyclonal antibody targeting human beta actin for use in WB, IHC, and ICC applications. Unconjugated liquid form with high specificity and broad species reactivity. Ideal as a loading control for protein detection assays.
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- PA585490
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Thermo Fisher Scientific beta Actin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta actin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.24 mg/mL |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792630 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%).
- Positive Control: 293T, A431, HeLa, HepG2, NIH-3T3.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein expressed in all human cell types and plays a key role in cell motility, structure, and integrity.
Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) in a two-stranded helical structure.
Due to its ubiquitous expression, beta actin is commonly used as a loading control in protein detection assays such as Western blotting.
Antibodies to beta actin are valuable tools for studying cytoskeletal organization and dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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