Thermo Fisher Scientific CD272 (BTLA) Monoclonal Antibody (6F7), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Biotin
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The BTLA antibody (clone 6F7) reacts with mouse BTLA (B and T lymphocyte attenuator). BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow, developing T cells in the thymus, and mature dendritic cells (not immature bone marrow-derived DCs). It functions as a negative regulator of activation and/or function in various hematopoietic cell types. BTLA is reported to bind B7-H4, though this interaction requires further study.

Clone-specific notes:
The 6F7 clone stains CD4⁺ and CD8⁺ single-positive thymocytes. Other clones (8F4, 3F9.D12, 6G3, 6H6) do not stain these cells but show similar staining in splenocytes and bone marrow cells.

Applications Reported:

• Flow cytometric analysis
• Blocking BTLA ligand binding

Applications Tested:

• Tested by flow cytometric analysis of mouse splenocytes
• Recommended ≤ 0.25 µg/test (in 100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test (empirical optimization recommended)

Storage and Handling:

• Use in sterile environment
• 0.2 µm post-manufacturing filtered

Purity and Quality:

• Purity: >90% (SDS-PAGE)
• Endotoxin level: <0.001 ng/µg antibody (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

BTLA (B and T-lymphocyte attenuator):
BTLA is a co-inhibitory receptor in the CD28 superfamily. It negatively regulates lymphocyte activation during immune responses. BTLA is constitutively expressed on most CD4⁺ and CD8⁺ T cells and decreases upon activation, remaining on Th1 but not Th2 cells. It interacts with HVEM (Herpesvirus Entry Mediator), a TNF receptor superfamily member, forming a key pathway in lymphocyte regulation and immune homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.