Thermo Fisher Scientific B7-H4 Monoclonal Antibody (H74), Functional Grade, eBioscience

Thermo Fisher Scientific B7-H4 Monoclonal Antibody (H74), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats:

• Unconjugated
• APC
• Biotin
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The H74 monoclonal antibody reacts with human B7-S1, also known as B7-H4 and B7x. Cross-reactivity with other proteins has not been determined. B7S1 is a newly discovered member of the B7 family and may regulate T cell costimulation depending on B cell activation status. While BTLA is reported as a counter receptor for B7S1, further studies are needed to confirm the B7-H4 ligand.

H74 stains human B7-H4 transfected cells but not peripheral blood cells. The expression pattern of B7-H4 is not fully characterized.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on transient hB7-H4 transfected cells. Recommended ≤0.25 µg/test (100 µL final volume). Optimal titration required.

Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

B7-H4 (B7x, VTCN1) is a costimulatory protein functioning as a negative regulator of T-cell mediated immunity. Although its receptor is unknown, it delivers inhibitory signals to T-cells, reducing proliferation, cell cycle progression, and IL-2 production.
B7-H4 is expressed on activated T-cells, B-cells, monocytes, and dendritic cells. Aberrant expression is linked to lung, breast, and ovarian cancers. Overexpression may suppress T-cell immunity and promote tumor progression.
VTCN1 may serve as a biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.