Thermo Fisher Scientific HLA-BC Monoclonal Antibody (B1.23.2), Functional Grade, eBioscience
HLA-B 및 HLA-C 항원을 인식하는 기능 등급 단클론 항체로, 유세포분석 및 기능적 억제 실험에 적합합니다. 높은 순도와 낮은 엔도톡신 수준을 보유하며, 4°C에서 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 1 publication |
| Functional Assay (Functional) | Assay-Dependent | View 1 publication |
| Inhibition Assays (IA) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B1.23.2 |
| Conjugate | Functional Grade |
| Available Formats | APC, PE, Request custom conjugation |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2866114 |
| Filtration | 0.2 µm post-manufacturing filtered |
| Purity | >90% (SDS-PAGE) |
| Endotoxin Level | <0.001 ng/µg antibody (LAL assay) |
| Aggregation | <10% (HPLC) |
Product Specific Information
Description:
The B1.23.2 monoclonal antibody recognizes human Human Leukocyte Antigens (HLA) B and C, which along with HLA-A correspond to MHC Class I proteins in humans. HLA-B and HLA-C are expressed on most cell types and form heterodimers with beta-2 microglobulin on the cell surface. These complexes are essential for presenting endoplasmic reticulum-derived peptides to immune cells. The antibody has been shown to inhibit binding of KIR2DL1 and LIR1.
Applications Reported:
This antibody is reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use ≤0.5 µg per test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Optimal performance requires titration for each assay.
Target Information
HLA-class I antigens are widely distributed on human nucleated cells. Expression levels may change under pathological conditions — for example, malignant cells may lose HLA-ABC, while hepatocytes in alcoholic hepatitis, biliary cirrhosis, and chronic active hepatitis may show enhanced expression.
HLA and MHC antibodies are critical tools in Immunopeptidomics, aiding in the identification and characterization of neoantigens using high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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