Thermo Fisher Scientific HLA-BC Monoclonal Antibody (B1.23.2), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

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Functional Assay (Functional)

Assay-Dependent

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Inhibition Assays (IA)

-

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Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B1.23.2

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• APC
• PE
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866114

Product Specific Information

Description: This B1.23.2 monoclonal antibody recognizes the human Human Leukocyte Antigens (HLA) B and C. Along with HLA-A, these proteins correspond to MHC Class I in humans. HLA-B and HLA-C are expressed on nearly all cell types and heterodimerize with beta-2 microglobulin at the cell surface. These molecular complexes play a critical role in the presentation of endoplasmic reticulum-derived peptides to immune cells. The antibody has been shown to inhibit binding of KIR2DL1 and LIR1.

Applications Reported: This B1.23.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B1.23.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.