Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), eFluor 450, eBioscience

Applications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV & Violet series (395–805 nm)
• eFluor 506, eFluor 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright series (436–780)
• Request custom conjugation

Product Specific Information

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except terminally differentiated plasma cells. Follicular dendritic cells also express CD19.
CD19 forms a multimolecular complex with CD21, CD81, and MHC class II, associating with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; recommended ≤0.5 µg/test (100 µL volume).
Cell number may range from 10⁵–10⁸ cells/test. Titration recommended for optimal performance.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and some myeloid leukemia cells.
CD19 serves as a key regulator of B cell activation, proliferation, and differentiation.
It lowers the threshold for BCR-dependent stimulation and can signal independently of BCR co-ligation.
Mutation of CD19 causes hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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