Thermo Fisher Scientific Cytokeratin 5/8 Monoclonal Antibody (RCK102)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

1:100-1:200

-

Product Specifications

Species Reactivity

Dog, Cat, Hamster, Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RCK102

Immunogen

Cytokeratins from human lung cancer cell line MR21. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 5/8,uniProtId:P05787-1,ncbiNodeId:9606,antigenRange:1-483,antigenLength:483,antigenImageFileName:MA1-06314_Cytokeratin_58_P05787-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06314_Cytokeratin_58_P05787-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Cytokeratin 5/8,uniProtId:P13647-1,ncbiNodeId:9606,antigenRange:1-590,antigenLength:590,antigenImageFileName:MA1-06314_Cytokeratin_58_P13647-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06314_Cytokeratin_58_P13647-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559785

Product Specific Information

MA1-06314 detects cytokeratin 5 and 8 in human, mouse, rat, rabbit, hamster, canine, swine, and feline samples.

MA1-06314 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry and immunohistochemistry applications.

The MA1-06314 immunogens are cytokeratins from a human lung cancer cell line (MR21).

Store at 4ºC or in small aliquots at -20ºC.

Target Information

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.