Thermo Fisher Scientific Mitotic Cells Monoclonal Antibody (8B3G)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:50-1:100

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Zebrafish

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

8B3G

Immunogen

Total cell lysate of the human bladder carcinoma cell line T24.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560123

Product Specific Information

MA1-06200 detects Mitotic cells in human samples.

MA1-06200 has sucessfully been used in immunocytochemistry and FACS procedures.

The MA1-06200 immunogen is total cell lysate of the human bladder carcinoma cell line T24.

Target Information

The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent` phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.