Thermo Fisher Scientific Phospho-Histone H3 (Thr3) Polyclonal Antibody
Phospho-Histone H3 (Thr3) 폴리클로날 항체로, Western blot에 적합하며 인간, 마우스, 랫트 시료에 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 버퍼에 보존됩니다. 세포 신호전달 및 염색질 연구에 활용됩니다.
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Applications and Tested Dilution
- Western Blot (WB): 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H3 (phospho Thr3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.38 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792792 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Chimpanzee (100%).
- Positive Control: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, HeLa (untreated), HeLa (0.1 µM Calyculin treatment for 30 minutes + phosphatase inhibitor).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or enable binding of specific transcription factors. The N-terminal tail of histone H3 can undergo methylation, acetylation, and phosphorylation, influencing various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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