Thermo Fisher Scientific Claudin 3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminus of mouse Claudin-3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Claudin 3,uniProtId:Q9Z0G9-1,ncbiNodeId:10090,antigenRange:219,antigenLength:219,antigenImageFileName:PA1-37469_Claudin_3_Q9Z0G9-1_Rabbit.svg,antigenImageFileNamePDP:PA1-37469_Claudin_3_Q9Z0G9-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2082840

Product Specific Information

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is small intestine tissue.

Target Information

Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.