Thermo Fisher Scientific Claudin 3 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

This antibody is predicted to react with bovine, canine, mouse, porcine, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is small intestine tissue.

Target Information

Claudin 1–8 proteins are a family of transmembrane proteins associated with tight junctions.
Tight junctions are specialized regions of cell-to-cell contact that act as molecular gaskets to prevent leakage of ions and water between cells.
They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity.
Different tissues exhibit different Claudin compositions.
Differential expression of this gene has been observed in various malignancies, including breast, ovarian, hepatocellular, urinary, prostate, lung, head and neck, and thyroid cancers.
Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.