Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor 780, eBioscience
마우스 Ly-6G(Gr-1)를 인식하는 단클론 항체로, 호중구 표지 및 유세포분석에 적합합니다. APC-eFluor 780 형광체 결합으로 적색 레이저(633–647 nm)에서 방출(780 nm)됩니다. 4°C 암소 보관, 동결 금지. 연구용 전용.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor 780, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 23 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | APC-eFluor™ 780 |
| Excitation/Emission Max | 756/785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2802290 |
Available Formats
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 395, 563, 661, 737
- Brilliant Violet 421, 480, 650, 786
- eFluor 450
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 702
- Request custom conjugation
Product Specific Information
Description:
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays a role in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a/CD11b. The ligand for Ly-6G is currently unknown.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended use ≤0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.
Fluorochrome Characteristics:
APC-eFluor 780 emits at 780 nm and is excited by the red laser (633–647 nm). Ensure the instrument supports this fluorochrome.
Light Sensitivity:
Tandem dye sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D):
Component of the myeloid differentiation antigen Gr-1, together with Ly6C. Molecular weight ~21–25 kDa, expressed by myeloid-derived cells in bone marrow. Marker for peripheral neutrophils, monocytes, and granulocytes. Expression correlates with granulocyte differentiation and maturation. Involved in anti-tumor response and may inhibit local immune responses through neutrophil interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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