Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor 780, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395, 563, 661, 737
• Brilliant Violet 421, 480, 650, 786
• eFluor 450
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 702
• Request custom conjugation

Product Specific Information

Description:
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays a role in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a/CD11b. The ligand for Ly-6G is currently unknown.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended use ≤0.25 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorochrome Characteristics:
APC-eFluor 780 emits at 780 nm and is excited by the red laser (633–647 nm). Ensure the instrument supports this fluorochrome.

Light Sensitivity:
Tandem dye sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser

Target Information

Ly6G (Lymphocyte antigen 6 complex locus G6D):
Component of the myeloid differentiation antigen Gr-1, together with Ly6C. Molecular weight ~21–25 kDa, expressed by myeloid-derived cells in bone marrow. Marker for peripheral neutrophils, monocytes, and granulocytes. Expression correlates with granulocyte differentiation and maturation. Involved in anti-tumor response and may inhibit local immune responses through neutrophil interaction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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