Thermo Fisher Scientific Ly-6G Recombinant Rat Monoclonal Antibody (1A8)
Recombinant rat monoclonal antibody (clone 1A8) specific for murine Ly6G. Ideal for flow cytometry and functional assays. High purity (Protein A), unconjugated form in PBS buffer. Reliable marker for granulocyte differentiation and maturation studies.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: Assay-dependent
Functional Assay (Functional)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1A8 |
| Immunogen | LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074326 |
Product Specific Information
Specificity: Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation and maturation. Together with Ly6C, it forms part of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, clone 1A8 does not bind Ly6C.
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, along with Ly6C. The Ly6G protein (~21–25 kDa) is expressed by myeloid-derived cells in bone marrow and serves as a marker for peripheral neutrophils, monocytes, and granulocytes. Gr-1 expression correlates with granulocyte differentiation and maturation. Although the physiological role of Ly6G remains unclear, studies suggest involvement in anti-tumor responses and inhibitory effects on local immune responses via neutrophil-mediated mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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