Thermo Fisher Scientific HMGB1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, HepG2, mouse brain, PC-12, Rat2, rat brain
• Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (92%), Dog (100%), Pig (99%), Rabbit (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HMGB1 (High-mobility group box-1) is a nuclear non-histone DNA-binding protein that also functions as a proinflammatory cytokine when released extracellularly by damaged or necrotic cells.
Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains: two positively charged DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162) and a negatively charged 30 aa C-terminal acidic tail.
Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The B box mediates cytokine activity, while the A box interacts with transcription factors.
HMGB1 acts as an inflammatory mediator promoting monocyte migration and cytokine secretion, and mediates T cell–dendritic cell interactions.
It signals through receptors such as RAGE, TLR2, and TLR4, and is highly conserved across species.
Elevated HMGB1 levels are associated with sterile tissue injury, infection, sepsis, arthritis, and ischemia-reperfusion injury.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.