
Thermo Fisher Scientific Histone H3.3 Polyclonal Antibody
Thermo Fisher Scientific의 Histone H3.3 Polyclonal Antibody는 다양한 종에서 높은 반응성을 보이는 Rabbit IgG 기반 항체입니다. Western blot, IHC, ICC/IF, ChIP 등 여러 응용에 적합하며, 재조합 단백질 항원으로 제작되었습니다. 연구용으로만 사용 가능합니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| ChIP assay (ChIP) | 1.5 µg/1×10^6 cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 1–136 of Human Histone H3.3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.74 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2547078 |
Product Specific Information
- Recommended positive controls: Jurkat, Raji, K562, THP-1, HL-60, NCI-H929, NIH-3T3, JC, BCL-1, C2C12, Raw264.7, PC-12, rice leaf
- Predicted reactivity: Mouse (100%), Zebrafish (99%), Xenopus laevis (100%), Pig (100%), Rabbit (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between nucleosomes to form higher-order chromatin structures.
This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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