Thermo Fisher Scientific Cytokeratin 14 Polyclonal Antibody
Human Cytokeratin 14 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P) 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 0.64 mg/mL 농도의 액상 형태. 4°C 단기, -20°C 장기 보관 권장.
- 카탈로그번호
- PA529608
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 197–433 of Human Cytokeratin 14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.64 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547084 |
Product Specific Information
- Recommended positive controls: 293T, A431, Jurkat, Raji
- Predicted reactivity: Mouse (96%), Rat (96%), Dog (96%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, present in both keratinizing (e.g., skin) and non-keratinizing (e.g., mesothelial) tissues. At least 20 different CKs have been identified.
CKs are classified into two types:
- Type I (acidic polypeptides)
- Type II (basic polypeptides)
Cytokeratin 14 belongs to the type I subfamily of low molecular weight keratins and pairs with keratin 5. It serves as a marker distinguishing stratified epithelial cells from simple epithelial cells and is useful for identifying squamous cell carcinomas. It is also considered a prognostic marker in breast carcinomas. Each epithelial cell expresses at least one acidic and one basic keratin family member.
Safety and Regulatory Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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