Thermo Fisher Scientific Phospho-DAXX (Ser668) Polyclonal Antibody
DAXX 단백질의 Ser668 인산화 부위를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot, IHC, ICC/IF에 적합. 인간 및 마우스 시료 반응성. 액상형, 1 mg/mL 농도, PBS/glycerol buffer에 보존.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DAXX (Accession Q9UER7), corresponding to amino acid residues around phosphorylated Ser668. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2818413 |
Product Specific Information
This antibody detects endogenous levels of DAXX only when phosphorylated at Ser668.
Target Information
The DAXX gene encodes a multifunctional protein localized in both the nucleus and cytoplasm. It interacts with various proteins including apoptosis antigen Fas, centromere protein C, and transcription factor E26 oncogene homolog 1. In the nucleus, DAXX acts as a transcriptional repressor binding to sumoylated transcription factors. Its repression can be relieved by sequestration into promyelocytic leukemia nuclear bodies or nucleoli. DAXX also associates with centromeres during G2 phase and may regulate apoptosis in the cytoplasm. Its localization and function are modulated by post-translational modifications such as sumoylation, phosphorylation, and polyubiquitination. Multiple transcript variants exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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