Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 (~30 kDa) is expressed on the cell surface as a disulfide-linked dimer and is rapidly upregulated upon activation, being expressed on lymphocytes, monocytes, and platelets.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

CellBlox Plus Blocking Buffer Usage:

• Required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample (regardless of the number of NovaFluors).
• For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton™ technology utilizing novel nucleic acid dye structures for engineered fluorescent signatures with reduced spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. CD69 expression is induced upon T lymphocyte activation and may play a role in proliferation and signal transduction in NK cells and platelets. Alternative splicing results in multiple transcript variants. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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