Thermo Fisher Scientific CD23 Monoclonal Antibody (EBVCS2), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant UV 805
• eFluor 450
• FITC
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells, and at low levels on T, NK, Langerhans cells, and platelets. Expression is upregulated upon B cell activation, and soluble forms are biologically active. CD23 functions as a low-affinity IgE receptor involved in IgE regulation and B cell activation. CD21 and the α subunits of CD11b/CD11c bind to CD23.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry using normal human peripheral blood cells.
  Recommended use: 5 µL (0.6 µg) per test in 100 µL final volume.
  Suitable for 10⁵–10⁸ cells/test.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining and should be added to antibody cocktails prior to combining with cells.
  • Add 5 µL per sample regardless of panel size.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates use Phiton™ technology, featuring engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD23 is a 45 kDa glycoprotein expressed on subsets of peripheral blood and tonsil B cells and strongly on EBV-transformed B lymphoblasts. It serves as the low-affinity IgE receptor on B cells and is involved in B cell growth, differentiation, and IgE production. Soluble CD23 acts as a mitogenic factor. CD23 expression is associated with conditions such as chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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