Thermo Fisher Scientific FGF9 Polyclonal Antibody, PeproTech
Recombinant Murine FGF-9을 항원으로 면역한 토끼에서 생산된 다클론 항체로, Western blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 동결건조 형태로 제공됩니다. 연구용으로 glial cell 성장 및 발달 연구에 활용됩니다.
- 카탈로그번호
- 500-P66-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FGF9 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine FGF-9 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930689 |
Product Specific Information
AA Sequence of recombinant protein:
PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine FGF-9. Anti-Murine FGF-9-specific antibody was purified by affinity chromatography employing an immobilized Murine FGF-9 matrix.
Sandwich ELISA:
To detect Murine FGF-9 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine FGF-9 (500-P66Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine FGF-9.
Western Blot:
To detect mFGF-9 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant mFGF-9 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene belongs to the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in various biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion.
This protein acts as a secreted factor that stimulates growth in cultured glial cells. In the nervous system, it is mainly produced by neurons and may play a crucial role in glial cell development. Expression of the mouse homolog is dependent on Sonic hedgehog (Shh) signaling. Mice lacking this gene exhibit male-to-female sex reversal, suggesting its role in testicular embryogenesis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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