Thermo Fisher Scientific GM-CSF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein
MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Murine GM-CSF. Anti-Murine GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA
To detect mGM-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine GM-CSF (500-P65) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of recombinant mGM-CSF.

Western Blot
To detect mGM-CSF by Western Blot, use at 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for recombinant mGM-CSF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging
500-P65BT-1MG is provided as 2 × 500 µg.

Target Information

The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes.
The active form exists as a homodimer in the extracellular space.
This gene is located in a cluster at chromosome region 5q31, associated with interstitial deletions in 5q- syndrome and acute myelogenous leukemia.
Other genes in the cluster include IL-4, IL-5, and IL-13.
The gene promotes tissue inflammation, and elevated cytokine levels have been observed in SARS-CoV-2 patients with acute respiratory distress syndrome.
Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.