Thermo Fisher Scientific TNF alpha Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific TNF alpha Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine TNF-alpha. Anti-Murine TNF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
For detection of Murine TNF-alpha, use 0.25–1.0 µg/mL antibody solution (100 µL/well). In combination with PeproTech Polyclonal Anti-Murine TNF-alpha (500-P64G) as capture antibody, detects ≥0.2–0.4 ng/well Recombinant Murine TNF-alpha.

Western Blot:
Use at 0.1–0.2 µg/mL for detection of Murine TNF-alpha. When used with compatible secondary reagents, detection limit is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P64GBT-1MG is supplied as 2 × 500 µg.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
Functions include:

• Regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
• Exists as multimeric noncovalent units (17 kDa monomer under SDS-PAGE)
• Involved in immune responses to bacterial, viral, parasitic, and fungal infections
• Plays a role in tumor necrosis and neuroprotection
• Implicated in autoimmune diseases, insulin resistance, and cancer

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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