Thermo Fisher Scientific GM-CSF Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine GM-CSF. The anti-Murine GM-CSF antibody was purified by affinity chromatography using an immobilized Murine GM-CSF matrix.

Sandwich ELISA:
To detect mGM-CSF by sandwich ELISA (100 µL/well), use 0.5–2.0 µg/mL of this antibody. When used with PeproTech Biotinylated Anti-Murine GM-CSF (500-P65Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant mGM-CSF.

Western Blot:
To detect mGM-CSF by Western blot, use 0.1–0.2 µg/mL of this antibody. With compatible secondary reagents, the detection limit for Recombinant mGM-CSF is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active protein exists as a homodimer in the extracellular space. This gene is located in a cluster at chromosome region 5q31, associated with interstitial deletions in 5q- syndrome and acute myelogenous leukemia. The cluster also includes interleukins 4, 5, and 13.
GM-CSF is involved in promoting tissue inflammation, and elevated cytokine levels have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.