Thermo Fisher Scientific CD337 (NKp30) Monoclonal Antibody (AF29-4D12), PE-Cyanine7, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

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Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AF29-4D12

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

View additional formats

• eFluor 450
• PE
• Request custom conjugation

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573446

Product Specific Information

Description: This AF29-4D12 monoclonal antibody reacts with human CD337, which is also known as NKp30. Expressed on NK cells, CD337 is the predominant member of the natural cytotoxicity receptor family that also includes NKp46 and NKp44. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells. Several ligands for this receptor have been reported, including B7-H6.

The AF29-4D12 antibody has been reported to crossreact to rhesus macaque.

Applications Reported: This AF29-4D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF29-4D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5` untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.