Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), PE-Cyanine7, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.25 µg/test
• View 5 publications

Product Specifications

Available Formats

• Alexa Fluor 488
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse (<0.5% of splenocytes) and is upregulated upon IFN-inducing stimulation. PDCA-1 cycles between surface and intracellular compartments and may regulate cytokine trafficking.

The eBio927 clone recognizes a distinct epitope from eBio129c, allowing co-staining and purification of pDCs.
Reported for use in flow cytometric analysis of mouse splenocytes. Recommended ≤0.25 µg/test (100 µL final volume). Optimize titration empirically for best performance.

• Light sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
• Excitation/Emission: 488–561 nm / 775 nm; compatible lasers: Blue, Green, Yellow-Green.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein involved in B-cell development and growth. It is expressed in B-cells, T-cells, monocytes, NK cells, DCs, and myeloma cells. BST2 inhibits retrovirus release from human cells, antagonized by HIV-1 Vpu. Its depletion eliminates Vpu dependence for viral release, suggesting potential therapeutic relevance for HIV/AIDS. BST2 dysfunction is also associated with stomatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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