Thermo Fisher Scientific Phospho-SMAD1/SMAD9 (Ser463, Ser465) Polyclonal Antibody
SMAD1/SMAD9의 Ser463/Ser465 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(Paraffin) 적용 가능. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-SMAD1/SMAD9 (Ser463, Ser465) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SMAD1 (Accession Q15797), corresponding to amino acid residues around phosphorylated Ser465 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817551 |
Product Specific Information
Antibody detects endogenous levels of Smad1/9 only when phosphorylated at Ser463/Ser465.
Target Information
The protein encoded by this gene belongs to the SMAD family, similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
This protein mediates bone morphogenetic protein (BMP) signaling, which regulates cell growth, apoptosis, morphogenesis, development, and immune responses. Upon BMP ligand stimulation, it is phosphorylated and activated by BMP receptor kinase, forming a complex with SMAD4 for transcriptional regulation.
It is also targeted by SMAD-specific E3 ubiquitin ligases (SMURF1, SMURF2) and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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