Thermo Fisher Scientific Phospho-SHP2 (Tyr542) Recombinant Rabbit Monoclonal Antibody (SN61-01)
SHP2 단백질의 Tyr542 인산화 부위를 특이적으로 인식하는 재조합 토끼 단클론 항체. Western blot 및 면역형광 분석에 적합. 높은 특이성과 재현성을 제공하며, 단백질 신호전달 연구에 유용. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN61-01 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr542 of Human SHP2 (aa 511–560) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809559 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Excellent specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the large rabbit immune repertoire
Target Information
The protein encoded by this gene belongs to the protein tyrosine phosphatase (PTP) family. PTPs act as signaling molecules regulating processes such as cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 (SH2) domains that mediate interactions with phospho-tyrosine residues of substrates.
It is widely expressed in most tissues and plays a regulatory role in various signaling events important for mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are associated with Noonan syndrome and acute myeloid leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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