
Thermo Fisher Scientific Nestin Recombinant Rabbit Monoclonal Antibody (SN06-27)
Recombinant rabbit monoclonal antibody recognizing human Nestin. Validated for WB, IHC, and ICC/IF. Offers high specificity, consistency, and sensitivity. Suitable for stem cell and neuroepithelial marker studies. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN06-27 |
| Immunogen | Synthetic peptide within Human Nestin (aa 531–580) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809558 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and individual clones are screened to select optimal candidates.
Key advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It serves as a key marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin is also a valuable angiogenic marker for assessing neovascularization in tumors. Specific antibodies to human Nestin aid in:
- Characterizing progenitor cell differentiation
- Enhancing therapeutic potential of neural stem/progenitor cells for CNS disease or injury
- Identifying neuroepithelial tumor cells
Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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