Thermo Fisher Scientific IL-4 Monoclonal Antibody (8D4-8), PE, eBioscience
Human IL-4 단백질을 검출하는 Mouse IgG1 모노클로날 항체로, PE 형광표지되어 Flow Cytometry 및 ELISA에 적합합니다. 488–561 nm에서 여기되고 578 nm에서 방출되며, 4°C 암소 보관이 필요합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific IL-4 Monoclonal Antibody (8D4-8), PE, eBioscience™
Applications
- Flow Cytometry (intracellular staining)
- Capture antibody for human IL-4 ELISA
Tested Dilution
- 5 µL (0.125 µg)/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Nematode |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D4-8 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1548823 |
Additional Formats
Product Specific Information
The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15–19 kDa cytokine secreted by Th2 cells.
It has been reported for use as a capture antibody for human IL-4 ELISA and for intracellular staining in flow cytometric analysis.
Pre-titrated and tested by intracellular staining for flow cytometry. Recommended test size: 5 µL (0.125 µg) per test (100 µL final volume).
Applicable for 10⁵–10⁸ cells/test (to be determined empirically).
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Post-manufacturing filtration: 0.2 µm.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and acts as a ligand for the IL-4 receptor.
IL-4 and IL-13 share overlapping functions due to receptor cross-binding.
STAT6 mediates the immune regulatory signaling pathway of IL-4.
IL-4 gene is part of a cytokine gene cluster on chromosome 5q, located near IL13, IL3, IL5, and CSF2.
IL-4δ2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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