Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet 421, eBioscience

Applications

Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Functional Grade
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal rat IgG2a kappa is useful as an isotype control immunoglobulin.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes and human peripheral blood cells; use at the same concentration as the experimental antibody.
• Fluorochrome: Brilliant Violet™ 421 (BV421) emits at 423 nm; designed for cytometers with a 405 nm violet laser.
• Recommendation: When using multiple polymer dye–conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

Rat IgG2a is an immunoglobulin subclass commonly used in research and diagnostics.
It is produced by the rat immune system and is known for:

• Activating complement
• Mediating antibody-dependent cell-mediated cytotoxicity (ADCC)

Molecular weight: ~160 kDa (two heavy chains + two light chains)
Applications: Control/reference antibody in rat model experiments, flow cytometry, immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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