Thermo Fisher Scientific AIRE Monoclonal Antibody (5H12), eFluor 660, eBioscience
마우스 AIRE 단백질을 인식하는 5H12 단클론 항체로, eFluor 660 형광 표지형입니다. IHC(F), ICC/IF, Flow Cytometry 등에 사용 가능합니다. 친화 크로마토그래피로 정제되었으며, 4°C 어두운 곳에 보관합니다. 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 5 |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | 3 |
| Flow Cytometry (Flow) | Assay-dependent | 9 |
| ChIP assay (ChIP) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5H12 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574256 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Custom conjugation available
Product Specific Information
This 5H12 monoclonal antibody reacts with mouse Autoimmune Regulator (AIRE), a 58-kDa transcription factor highly expressed in mature medullary thymic epithelial cells (mTECs). Although AIRE mRNA is detected in peripheral tissues such as lymph nodes and dendritic cells, protein expression outside the thymus remains unclear.
AIRE is involved in thymocyte development and regulation of tissue-specific antigen expression during negative selection. It also contributes to chemokine expression and antigen presentation.
The 5H12 antibody recognizes mouse AIRE isoforms 1 and 2.
Applications Reported:
Used for intracellular flow cytometric analysis and immunohistochemical staining of frozen tissue sections.
Applications Tested:
Tested by immunohistochemistry on frozen 5-week-old mouse thymus; recommended ≤10 µg/mL. Optimize dilution for specific assays.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
AIRE is a transcriptional regulator that binds DNA as a dimer or tetramer and regulates gene expression. It is a nuclear protein composed of 545 amino acids, containing an HSR domain, two PHD-type zinc fingers, and a SAND domain. It may form homodimers, homotetramers, and nuclear bodies, interacting with coactivators CBP and CREBBP.
Three isoforms of AIRE are reported, one potentially involved in nuclear-mediated decay (NMD). AIRE mediates E3 ubiquitin ligase activity and plays a critical role in immune regulation. Mutations in AIRE cause autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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