Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• eFluor 450
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines Ramos and Daudi. Strain variability exists, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety (Neu5Ac), recognized by CD22.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes, recommended ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, excited with red laser (633 nm).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GL7 is a cell-surface protein expressed on activated T and B lymphocytes, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines Ramos and Daudi. Expression varies between strains (BALB/c > C57BL/6). The GL7 epitope is a sialic acid glycan moiety (Neu5Ac) recognized by CD22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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