Thermo Fisher Scientific WNT4 Recombinant Rabbit Monoclonal Antibody (9H2L10)
WNT4 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot 및 면역세포염색에 적합. 높은 특이성과 민감도를 제공하며, 로트 간 일관성이 우수. 인간에 반응하며 원숭이, 돼지, 쥐, 생쥐에서도 예측 반응. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 9H2L10 |
| Immunogen | Multiple peptides corresponding to Human Wnt4 (aa 58–71; 235–253; 258–269) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2608445 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Rat, and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
The WNT gene family consists of structurally related genes encoding secreted signaling proteins involved in oncogenesis and developmental processes such as regulation of cell fate and patterning during embryogenesis.
WNT4 is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein with 98% amino acid identity to mouse and rat Wnt4, playing a key role in female development and prevention of testes formation.
WNT4, along with WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations can lead to Rokitansky-Kuster-Hauser syndrome and SERKAL syndrome.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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