Thermo Fisher Scientific DUSP1 Monoclonal Antibody (3A9)
DUSP1 단백질을 표적하는 Mouse IgG2b 단클론 항체로, WB, ICC/IF, ELISA에 적합. 인산화 MAP kinase를 비활성화하는 DUSP1 연구에 활용. PBS 기반 액상 형태, 보존제 무첨가, -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3A9 |
| Immunogen | DUSP1 (NP_004408, 305–367 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
LLQFESQVLA PHCSAEAGSP AMAVLDRGTS TTTVFNFPVS IPVHSTNSAL SYLQSPITTS PSC
Target Information
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors.
It encodes a protein structurally similar to non-receptor-type protein-tyrosine phosphatases and shows sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus.
The bacterially expressed and purified DUSP1 protein exhibits intrinsic phosphatase activity, specifically inactivating mitogen-activated protein (MAP) kinase by dephosphorylating both phosphothreonine and phosphotyrosine residues.
It suppresses MAP kinase activation by oncogenic ras in Xenopus oocyte extracts, suggesting its role in cellular stress response and negative regulation of proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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