Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (6F11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:40-1:80

View 7 publications 7 publications

Immunohistochemistry (Frozen) (IHC (F))

1:40-1:60

-

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Cat, Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6F11

Immunogen

Recombinant estrogen receptor protein (alpha form)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

< 0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_930763

Product Specific Information

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for this product is breast carcinoma.

Mouse anti Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERalpha), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.