Thermo Fisher Scientific CD11b Monoclonal Antibody (198)
토끼 CD11b 단백질을 인식하는 마우스 단클론 항체로, IHC, ICC, Flow Cytometry, IP 등 다양한 응용에 적합. 단백질 A로 정제된 액상 항체이며 1 mg/mL 농도로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:100–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 198 |
| Immunogen | Rabbit adherent blood leucocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745227 |
Product Specific Information
Mouse anti-Rabbit CD11b antibody, clone 198 recognizes the rabbit CD11b cell surface glycoprotein, also known as the integrin alpha M chain and MAC-1.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18).
The CD11b/CD18 heterodimeric complex (integrin alpha-M beta-2, Mac-1, CR3) functions as a receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
These interactions are critical for cell-cell and cell-matrix adhesion.
CD11b is expressed on:
- Monocytes/macrophages
- Granulocytes
- Activated lymphocytes
- Subsets of NK cells and dendritic cells
- Microglia in the brain
Expression levels vary by tissue and cell type, increasing during monocyte maturation. Peritoneal macrophages exhibit higher CD11b expression than splenic macrophages.
Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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