Thermo Fisher Scientific Phospho-JNK1/JNK2/JNK3 (Thr183, Tyr185) Polyclonal Antibody
JNK1/2/3 인산화(Thr183, Tyr185) 상태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 1 mg/mL 농도, PBS/glycerol buffer, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAPK8 (Accession P45983), corresponding to amino acid residues around phosphorylated Thr183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903058 |
Product Specific Information
This antibody detects endogenous levels of JNK1, JNK2, and JNK3 only when phosphorylated at Threonine 183 and Tyrosine 185.
Target Information
C-Jun N-terminal Kinase (JNK), also known as Mitogen-activated protein kinase (MAPK8), is a member of the MAPK superfamily of stress-activated protein kinases. MAPKs are serine-threonine kinases activated by various extracellular stimuli and mediate signal transduction from the cell surface to the nucleus. JNK is involved in numerous cellular processes such as proliferation, differentiation, transcription regulation, and development. JNK pathways are activated by stress and inflammatory signals. It is expressed as ten isoforms due to differential mRNA splicing, with JNK1 and JNK2 being predominant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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