Thermo Fisher Scientific Phospho-HSF1 (Ser303) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and are located upstream of all heat shock genes.

The HSEs are highly conserved among species; however, HSF purified from yeast, Drosophila, and human have different molecular weights and do not show significant immunological cross-reactivity. Two HSFs have been identified in human cells: HSF1 and HSF2, which share 38% sequence identity and bind to the same HSEs. HSF1 is activated by classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.

HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 becomes activated into a nuclear-localized trimeric state that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.