Thermo Fisher Scientific Connexin 43 Polyclonal Antibody
Connexin 43 단백질을 인식하는 Thermo Fisher Scientific의 고품질 폴리클로날 항체입니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합합니다. 사람, 마우스, 랫트 반응성 확인. KLH 결합 합성 펩타이드로 면역화된 토끼 IgG 항체로, 친화 크로마토그래피로 정제되었습니다.
- 카탈로그번호
- 600-401-MJ2x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Connexin 43 Polyclonal Antibody
Thermo Fisher Scientific Connexin 43 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:200 |
| Immunocytochemistry (ICC/IF) | 15 µg/mL |
| ELISA | 1:10,000–1:15,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal portion of mouse connexin 43 conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.92 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity-purified antibody is directed against rat connexin 43. BLAST analysis suggests cross-reactivity based on 100% homology with rat, black bear, European rabbit, wild boar, Macaca fascicularis, and Chlorocebus aethiops.
Target Information
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as hexamers to form hemichannels that pair with adjacent cell hemichannels to create gap junctions, enabling direct cell-to-cell communication. These junctions are essential for development and regulation of cell growth.
Phosphorylation of Cx43 by protein kinase C (PKC) at Ser368 reduces intercellular communication. Src kinase can also phosphorylate Cx43 to modulate gap junction function. Connexin 43 is the predominant gap junction protein in cardiac tissue, playing a key role in synchronized heart contraction and embryonic development. It is regulated by multiple kinases influencing myocardial cell coupling.
A connexin 43 pseudogene (GJA1P) is mapped to chromosome 5. Mutations in related genes can cause X-linked Charcot-Marie-Tooth disease, oculodentodigital dysplasia, and cardiac malformations. Alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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