Thermo Fisher Scientific CaMKII alpha Monoclonal Antibody (Cba-2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

-

View 5 publications 5 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

0.1-1 µg/mL

-

Immunoprecipitation (IP)

2 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fish, Human, Mouse, Sheep, Rat

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

Cba-2

Immunogen

Purified rat brain CaM Kinase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CaMKII alpha,uniProtId:P11275-1,ncbiNodeId:10114,antigenRange:1-478,antigenLength:478,antigenImageFileName:13-7300_CaMKII_alpha_P11275-1_House_mouse.svg,antigenImageFileNamePDP:13-7300_CaMKII_alpha_P11275-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533032

Product Specific Information

This antibody is specific for alpha subunit of CaM Kinase II. No significant reactivity was seen with the B, B1, yB, dA, dB, and dC isoforms. The epitope is located in the regulatory domain.

Target Information

CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.