Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (TU-01)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 33 publications 33 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1:250

View 11 publications 11 publications

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate, Pig, Protozoa, Rat, Virus, Xenopus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TU-01

Immunogen

Reacts with peptides that correspond to aa 65-79 in the N-terminus of alpha-tubulin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Tubulin,uniProtId:Q71U36-1,ncbiNodeId:9606,antigenRange:65-79,antigenLength:451,antigenImageFileName:13-8000_alpha_Tubulin_Q71U36-1_House_mouse.svg,antigenImageFileNamePDP:13-8000_alpha_Tubulin_Q71U36-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Caprylic acid precipitation, Ammonium sulfate precipitation, sequential chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533035

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.