Thermo Fisher Scientific 4-Hydroxy-2-hexenal Monoclonal Antibody (5C11.1), FITC
4-Hydroxy-2-hexenal을 특이적으로 인식하는 FITC 결합 단클론 항체. Western blot, ELISA, Dot blot에 적합. 498/517 nm 형광 특성. 단백질 G 정제, 4°C 암소 보관. 4-Hydroxynonenal 등 산화 스트레스 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:1,000 |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C11.1 |
| Immunogen | Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932312 |
Available Formats
- Unconjugated (MA5-27576)
- APC (MA5-45857)
- PE (MA5-45860)
- PerCP (MA5-45859)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45858 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal (4-HNE, C9H16O2) is an alpha,beta-unsaturated hydroxyalkenal produced by lipid peroxidation in cells. It is the primary alpha,beta-unsaturated hydroxyalkenal formed during this process and is widely used as a biomarker for oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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