Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), PE-Cyanine7, eBioscience

Applications and Tested Dilution

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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 496 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 555 / 585 / 610-30S / 660-40S / 690 / 760
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 570 / 590 / 660
• PE
• PE-Cyanine5
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600
• Request custom conjugation available

Product Specific Information

Description:
The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1, and MAdCAM-1, mediating lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) in peripheral lymphoid tissues.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation/Emission:
488–561 nm excitation; 775 nm emission.
Compatible with Blue, Green, and Yellow-Green lasers.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

The human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor. It includes:

• N-terminal C-type lectin binding domain
• EGF-like domain
• Two short consensus repeat (SCR) sequences
• Spacer, transmembrane, and cytoplasmic regions

CD62L is constitutively expressed on most leukocytes, including lymphocytes (except some memory T cells), monocytes, and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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