Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), PE-Cyanine7, eBioscience
인체 CD62L(L-Selectin)을 인식하는 DREG-56 단클론 항체로, PE-Cyanine7으로 표지되어 유세포분석(Flow Cytometry)에 적합합니다. 5 µL/test로 사용 가능하며, 488–561 nm에서 여기되어 775 nm에서 방출됩니다. 4°C 어둠에서 보관하며, 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DREG-56 (DREG56) |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569/780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1257143 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Brilliant UV 496 / 615 / 737 / 805
- Brilliant Violet 421 / 480 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 555 / 585 / 610-30S / 660-40S / 690 / 760
- NovaFluor Red 710 / 725 / 755
- NovaFluor Yellow 570 / 590 / 660
- PE
- PE-Cyanine5
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600
- Request custom conjugation available
Product Specific Information
Description:
The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1, and MAdCAM-1, mediating lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) in peripheral lymphoid tissues.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission:
488–561 nm excitation; 775 nm emission.
Compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
The human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor. It includes:
- N-terminal C-type lectin binding domain
- EGF-like domain
- Two short consensus repeat (SCR) sequences
- Spacer, transmembrane, and cytoplasmic regions
CD62L is constitutively expressed on most leukocytes, including lymphocytes (except some memory T cells), monocytes, and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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