Thermo Fisher Scientific alpha Actinin 4 Polyclonal Antibody
Human, Mouse, Rat 반응성을 가진 alpha Actinin 4 다클론 항체로 WB, IHC, ICC/IF, IP에 사용 가능. Rabbit IgG 호스트로 제작되었으며 항원 친화 크로마토그래피로 정제. 액상 형태, PBS buffer에 0.1% sodium azide 포함, -20°C 보관.
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Applications 및 Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–3 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 2–3 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 2–3 µg/mL | View 4 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the N-terminal region of the human, mouse, and orangutan alpha-Actinin-4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533514 |
Target Information
Alpha actinins belong to the spectrin gene superfamily, representing a diverse group of cytoskeletal proteins including alpha and beta spectrins and dystrophins.
Alpha actinin is an actin-binding protein with multiple roles in different cell types.
In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, binding actin to the membrane.
In skeletal, cardiac, and smooth muscle, isoforms are localized to the Z-disc and analogous dense bodies, anchoring myofibrillar actin filaments.
This gene encodes a nonmuscle alpha actinin isoform concentrated in the cytoplasm and thought to be involved in metastatic processes.
Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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