Thermo Fisher Scientific Calreticulin precursor Polyclonal Antibody
Calreticulin precursor를 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 적합. 인간 단백질에 반응하며, 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 0.2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Calreticulin precursor affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human calreticulin precursor protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.42 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 50% glycerol |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
A BLAST analysis was used to suggest cross-reactivity with human, mouse, rat, primate, and chicken based on 100% sequence homology.
Cross-reactivity with Calreticulin precursor from other sources has not been determined.
Target Information
Calr (Calreticulin) is a calcium-binding chaperone that promotes folding, oligomeric assembly, and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle.
Calr, a lectin, interacts transiently with almost all monoglucosylated glycoproteins synthesized in the ER.
It also interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
Calr may be involved in maternal gene expression regulation and oocyte maturation through calcium homeostasis regulation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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